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	Provedor de dados BJM (1) BJPS (1) Autor Charousová,Ivana (2) Hleba,Lukáš (2) Javoreková,Soňa (2) Medo,Juraj (2) Císarová,Miroslava (1) Palavra-chave 16S rRNA (2) MALDI-TOF MS (2) Streptomyces (2) Actinomycin/antimicrobial activity (1) Factumycin (1) Soil (1) Tetrangomycin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2018 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa BJPS Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Císarová,Miroslava; Javoreková,Soňa. In the present study we reported the antimicrobial activity of actinomycetes isolated from aridic soil sample collected in Karoo, South Africa. Eighty-six actinomycete strains were isolated and purified, out of them thirty-four morphologically different strains were tested for antimicrobial activity. Among 35 isolates, 10 (28.57%) showed both antibacterial and antifungal activity. The ethyl acetate extract of strain KRG-1 showed the strongest antimicrobial activity and therefore was selected for further investigation. The almost complete nucleotide sequence of the 16S rRNA gene as well as distinctive matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (MALDI-TOF/MS) profile of whole-cell proteins acquired for strain KRG-1 led to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycin/antimicrobial activity; Streptomyces; Soil; 16S rRNA; MALDI-TOF MS. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100594 Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics BJM Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Javoreková,Soňa. ABSTRACT Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; 16S rRNA; MALDI-TOF MS; Tetrangomycin; Factumycin. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400816 Registros recuperados: 2 Primeira ... 1 ... Última

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